CRISPR-Cas9 SIMULATION

▸ Loading the double-stranded DNA & target segment…
▸ Designing the guide RNA (~20 nt)
▸ Assembling the Cas9 + gRNA complex
▸ Calibrating PAM (NGG) recognition
▸ Connecting the 2 repair pathways NHEJ / HDR
▸ Ready — Online. ✅
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⌂ Cells

Simulation room CRISPR & Gene Editing

CRISPR-Cas9 Gene Editing
Online
Cas9 · gRNA · PAM
Cut & repair progress
✂️ CRISPR-Cas9
Target match—
Cut—
Repair efficiency—
Off-target—
Scenario—
Viewing—
Notes
gRNA aims · Cas9 is the scissors · PAM is the safety lock. Cas9 only cuts when it sees a PAM (NGG) and the gRNA pairs closely enough → a double-strand break → the cell patches it (breaking the gene or inserting the correct one).
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Pick a "Scenario" (search / cut / knock-out / knock-in / off-target / therapy) · click each component or concept to see details
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Cumulative repair efficiency · on-target vs off-target per cycle on-target (cumulative)repair efficiencyoff-target (cumulative)
About CRISPR

CRISPR is one of 20 topics in the Cell & Genetics domain (10⁻⁵ m scale) at My Labs — an interactive simulation atlas. This virtual lab runs right in your browser: adjust the parameters, pick a scenario and watch the phenomenon change in real time, with a click-to-inspect cross-section for every structure.

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